国产农村妇女作爱视频播放,无码熟妇人妻av在线影片软件 ,末成年女a片一区二区,天体野营

您好!歡迎訪問上海起發(fā)實驗試劑有限公司網(wǎng)站!
全國服務(wù)咨詢熱線:

15921799099

當(dāng)前位置:首頁 > 資料下載 > Invitrogen GeneSwith 哺乳動物誘導(dǎo)系統(tǒng)pSwitch 說明書

Invitrogen GeneSwith 哺乳動物誘導(dǎo)系統(tǒng)pSwitch 說明書

發(fā)布時間:2013/7/22      點擊次數(shù):2639

 Invitrogen GeneSwith 哺乳動物誘導(dǎo)系統(tǒng)

Invitrogen

VectorspSwitch

產(chǎn)品參數(shù): 

Mammalian Selection:Hygromycin

載體抗性: 
氨芐青霉素(Ampicillin)
載體描述: 

Switch-On Expression from the Lowest Basal Levels

The GeneSwitch? System for inducible mammalian expression is ideal for experiments that require the absolute lowest uninduced expression levels. The expression vector pGene/V5-His provides a minimal promoter, GAL4-E1b, consisting of the binding sites for the yeast Gal4 DNA binding protein followed by the TATA sequence from the Adenovirus E1b promoter. Without additional factors, the GAL4-E1b promoter is transcriptionally silent.

To activate transcription from the GAL4-E1b promoter, the GeneSwitch? regulatory protein is expressed from a minimal TK promoter on the pSwitch vector. The GeneSwitch? protein has three functional domains:

  1. Gal4 DNA Binding Domain (Gal4-DBD) to bind the regulatory protein to the GAL4-E1b promoter
  2. Truncated human Progesterone Receptor Ligand Binding Domain (hPR-LBD) that undergoes a conformational change when it binds the progesterone antagonist, mifepristone
  3. Transcription activation domain from the NF?B transcription factor p65 (p6D) to activate transcription from the silent GAL4-E1b promoter

In the absence of mifepristone, the conformation of the hPR-LBD region prevents the GeneSwitch? regulatory protein from activating transcription from the GAL4-E1b promoter. When mifepristone is added and binds the hPR-LBD region, the GeneSwitch? regulatory protein assumes a conformation that permits it to stimulate transcription from the GAL4-E1b promoter.

Induction of the GeneSwitch? System leads to activation of the gene of interest on the vector pGene/V5-His. In addition, four Gal4 binding sites upstream of the minimal HSV TK promoter on pSwitch can bind the pSwitch regulatory protein. Therefore, adding mifepristone up-regulates production of the regulatory protein. The increased levels of the GeneSwitch? regulatory protein result in induction of the gene of interest from pGene/V5-His to levels that can approach those of viral promoters.

The GeneSwitch? Kit offers exceptionally low uninduced and high induced expression levels in mammalian cells. The system provides the expression vector pGene/V5-His with the minimal, synthetic GAL4-E1b promoter that is transcriptionally silent until activated. The GeneSwitch? regulatory protein binds the GAL4-E1b promoter to activate transcription upon the addition of mifepristone. In addition, the GeneSwitch? protein upregulates its own expression, leading to amplified expression of the gene of interest from pGene/V5-His. pGene/V5-His offers several features that facilitate expression analysis and purification of recombinant proteins in mammalian cells:

  1. C-terminal V5 epitope tag for detection with Anti-V5 Antibodies
  2. C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with Anti-His(C-term) Antibodies
  3. The Zeocin? resistance gene for effective selection in both mammalian cells and E. coli

 


文件下載    
上海起發(fā)實驗試劑有限公司
地址:上海浦東川沙鎮(zhèn)川沙路6619號上海起發(fā)實驗試劑有限公司
郵箱:xs1@78bio.com
傳真:021-50724961
關(guān)注我們
歡迎您關(guān)注我們的微信公眾號了解更多信息:
歡迎您關(guān)注我們的微信公眾號
了解更多信息
亚洲午夜无码av毛片久久| 肉欲娇宠(h)皇帝篇31| 国产成人无码综合亚洲日韩| 久久久婷婷五月亚洲97号色| 日韩精品无码中文字幕一区二区| 性饥渴艳妇k8经典a片| 在线观看国产精品| 亚洲av日韩av无码| nba直播在线观看免费| 免费观看全黄做爰的视频| 男男黄GAY片免费网站WWW| 国产精品一区二区在线观看| 蜜桃av色偷偷av老熟女| 我高潮太爽忍不住大叫怎么办| 精品精品国产高清a级毛片| 免费观看又色又爽又黄的| 公与淑婷厨房猛烈进出| 成全视频在线观看免费高清| 中文字幕一区二区| 精品免费囯产一区二区三区四区| 军人全身脱精光自慰| 被黑人猛烈进出到抽搐| 18gay男同69亚洲帅男蓝宇| 国产69久久精品成人看| japanese55丰满成熟| 日韩无码av| 少妇无套内谢久久久久| 欧亚乱熟女一区二区在线| 国产女人18毛片水真多1| 国产在线av一区二区麻豆董小宛 | 天天人人爽人人爽人人爽动漫| 免费A级毛片| 一区二区三区| 色94色欧美sute亚洲线路二| 国产真实偷人视频| 色情污污污www网站下载| 国产亚洲av片在线观看18女人| 亚洲av成人片色在线观看高潮| 一本一道人人妻人人妻αv| 苍井空被躁50分钟5分钟免费 | 亚洲一区二区观看播放|