国产农村妇女作爱视频播放,无码熟妇人妻av在线影片软件 ,末成年女a片一区二区,天体野营

您好!歡迎訪問上海起發(fā)實驗試劑有限公司網(wǎng)站!
全國服務(wù)咨詢熱線:

15921799099

當(dāng)前位置:首頁 > 資料下載 > Invitrogen pGene v5-His 說明書

Invitrogen pGene v5-His 說明書

發(fā)布時間:2013/7/22      點(diǎn)擊次數(shù):2250

 

GeneSwith 哺乳動物誘導(dǎo)系統(tǒng)

Invitrogen  pGene v5-His 

 

 

產(chǎn)品參數(shù): 

Mammalian Selection:Zeocin
Constitutive/Inducible:Inducible
Expression Level Tightly controlled (activate with mifepristone)
Sequencing Primer pGene Fwd 
Sequencing Primer Sequence:5'd[CTGCTATTCTGCTCAACCT]3' 
Protein Tags:6X His,V5

載體抗性: 
氨芐青霉素(Ampicillin)
載體描述: 

Switch-On Expression from the Lowest Basal Levels

The GeneSwitch™ System for inducible mammalian expression is ideal for experiments that require the absolute lowest uninduced expression levels. The expression vector pGene/V5-His provides a minimal promoter, GAL4-E1b, consisting of the binding sites for the yeast Gal4 DNA binding protein followed by the TATA sequence from the Adenovirus E1b promoter. Without additional factors, the GAL4-E1b promoter is transcriptionally silent.

To activate transcription from the GAL4-E1b promoter, the GeneSwitch™ regulatory protein is expressed from a minimal TK promoter on the pSwitch vector. The GeneSwitch™ protein has three functional domains:

  1. Gal4 DNA Binding Domain (Gal4-DBD) to bind the regulatory protein to the GAL4-E1b promoter
  2. Truncated human Progesterone Receptor Ligand Binding Domain (hPR-LBD) that undergoes a conformational change when it binds the progesterone antagonist, mifepristone
  3. Transcription activation domain from the NFØB transcription factor p65 (p6D) to activate transcription from the silent GAL4-E1b promoter

In the absence of mifepristone, the conformation of the hPR-LBD region prevents the GeneSwitch™ regulatory protein from activating transcription from the GAL4-E1b promoter. When mifepristone is added and binds the hPR-LBD region, the GeneSwitch™ regulatory protein assumes a conformation that permits it to stimulate transcription from the GAL4-E1b promoter.

Induction of the GeneSwitch™ System leads to activation of the gene of interest on the vector pGene/V5-His. In addition, four Gal4 binding sites upstream of the minimal HSV TK promoter on pSwitch can bind the pSwitch regulatory protein. Therefore, adding mifepristone up-regulates production of the regulatory protein. The increased levels of the GeneSwitch™ regulatory protein result in induction of the gene of interest from pGene/V5-His to levels that can approach those of viral promoters.

The GeneSwitch™ Kit offers exceptionally low uninduced and high induced expression levels in mammalian cells. The system provides the expression vector pGene/V5-His with the minimal, synthetic GAL4-E1b promoter that is transcriptionally silent until activated. The GeneSwitch™ regulatory protein binds the GAL4-E1b promoter to activate transcription upon the addition of mifepristone. In addition, the GeneSwitch™ protein upregulates its own expression, leading to amplified expression of the gene of interest from pGene/V5-His. pGene/V5-His offers several features that facilitate expression analysis and purification of recombinant proteins in mammalian cells:

  1. C-terminal V5 epitope tag for detection with Anti-V5 Antibodies
  2. C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with Anti-His(C-term) Antibodies
  3. The Zeocin resistance gene for effective selection in both mammalian cells and E. coli

 


文件下載    
上海起發(fā)實驗試劑有限公司
地址:上海浦東川沙鎮(zhèn)川沙路6619號上海起發(fā)實驗試劑有限公司
郵箱:xs1@78bio.com
傳真:021-50724961
關(guān)注我們
歡迎您關(guān)注我們的微信公眾號了解更多信息:
歡迎您關(guān)注我們的微信公眾號
了解更多信息
国产欧美熟妇另类久久久| 全黄裸片一29分钟免费真人版| 交换俱乐部娇妻奶呻吟啊视频| 男男做a爱过程免费视频| 狠狠躁夜夜躁人爽碰| 书生屁股被cao成sao货男男| 性之剧毒在线观看| 骚虎视频在线观看| 国产人与禽ZOZ0性伦| 国产又粗又大又硬又长又爽| 两根粗大在她腿间进进出出h| 狠狠躁天天躁夜夜躁婷婷| 把腿张开老子臊烂你| 日本无翼乌邪恶彩色大全| yw尤物av无码国产在线观看| 业余 自由 性别 视频 视频| 精品黑人一区二区三区久久| 男男gaygay无套gay无套| 丁香婷婷综合激情五月色| 粉嫩被黑人两根粗大猛烈进出视频| 18禁无码国内精品久久综合88| yy4080旧里番未删减版| 荫蒂添的好舒服视频囗交| 色综合久久网| 东北老妇爽大叫受不了| 一色一伦一区二区三区| 快穿之受收精子系统肉肉h| 成全动漫视频在线观看免费高清| 年轻丰满的继牳理伦片中文| 欧美+日韩+国产| 国产手机拍视频推荐2023| 日本爆乳强伦中文字幕电影| 亚洲国产精品无码| 免费又黄又爽又色的视频| 少爷托着娇乳撞击娇吟| 迈开腿让我看看你的草莓| 伦理片在线观看| 欧美精产国品一二三区别| 中文精品久久久久人妻不卡| 特黄特色大片免费播放器图片| 高潮aaa人人爽人人爱|