国产农村妇女作爱视频播放,无码熟妇人妻av在线影片软件 ,末成年女a片一区二区,天体野营

您好!歡迎訪問上海起發(fā)實驗試劑有限公司網(wǎng)站!
全國服務(wù)咨詢熱線:

15921799099

當(dāng)前位置:首頁 > 資料下載 > Invitrogen pGene v5-His 說明書

Invitrogen pGene v5-His 說明書

發(fā)布時間:2013/7/22      點(diǎn)擊次數(shù):2250

 

GeneSwith 哺乳動物誘導(dǎo)系統(tǒng)

Invitrogen  pGene v5-His 

 

 

產(chǎn)品參數(shù): 

Mammalian Selection:Zeocin
Constitutive/Inducible:Inducible
Expression Level Tightly controlled (activate with mifepristone)
Sequencing Primer pGene Fwd 
Sequencing Primer Sequence:5'd[CTGCTATTCTGCTCAACCT]3' 
Protein Tags:6X His,V5

載體抗性: 
氨芐青霉素(Ampicillin)
載體描述: 

Switch-On Expression from the Lowest Basal Levels

The GeneSwitch™ System for inducible mammalian expression is ideal for experiments that require the absolute lowest uninduced expression levels. The expression vector pGene/V5-His provides a minimal promoter, GAL4-E1b, consisting of the binding sites for the yeast Gal4 DNA binding protein followed by the TATA sequence from the Adenovirus E1b promoter. Without additional factors, the GAL4-E1b promoter is transcriptionally silent.

To activate transcription from the GAL4-E1b promoter, the GeneSwitch™ regulatory protein is expressed from a minimal TK promoter on the pSwitch vector. The GeneSwitch™ protein has three functional domains:

  1. Gal4 DNA Binding Domain (Gal4-DBD) to bind the regulatory protein to the GAL4-E1b promoter
  2. Truncated human Progesterone Receptor Ligand Binding Domain (hPR-LBD) that undergoes a conformational change when it binds the progesterone antagonist, mifepristone
  3. Transcription activation domain from the NFØB transcription factor p65 (p6D) to activate transcription from the silent GAL4-E1b promoter

In the absence of mifepristone, the conformation of the hPR-LBD region prevents the GeneSwitch™ regulatory protein from activating transcription from the GAL4-E1b promoter. When mifepristone is added and binds the hPR-LBD region, the GeneSwitch™ regulatory protein assumes a conformation that permits it to stimulate transcription from the GAL4-E1b promoter.

Induction of the GeneSwitch™ System leads to activation of the gene of interest on the vector pGene/V5-His. In addition, four Gal4 binding sites upstream of the minimal HSV TK promoter on pSwitch can bind the pSwitch regulatory protein. Therefore, adding mifepristone up-regulates production of the regulatory protein. The increased levels of the GeneSwitch™ regulatory protein result in induction of the gene of interest from pGene/V5-His to levels that can approach those of viral promoters.

The GeneSwitch™ Kit offers exceptionally low uninduced and high induced expression levels in mammalian cells. The system provides the expression vector pGene/V5-His with the minimal, synthetic GAL4-E1b promoter that is transcriptionally silent until activated. The GeneSwitch™ regulatory protein binds the GAL4-E1b promoter to activate transcription upon the addition of mifepristone. In addition, the GeneSwitch™ protein upregulates its own expression, leading to amplified expression of the gene of interest from pGene/V5-His. pGene/V5-His offers several features that facilitate expression analysis and purification of recombinant proteins in mammalian cells:

  1. C-terminal V5 epitope tag for detection with Anti-V5 Antibodies
  2. C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with Anti-His(C-term) Antibodies
  3. The Zeocin resistance gene for effective selection in both mammalian cells and E. coli

 


文件下載    
上海起發(fā)實驗試劑有限公司
地址:上海浦東川沙鎮(zhèn)川沙路6619號上海起發(fā)實驗試劑有限公司
郵箱:xs1@78bio.com
傳真:021-50724961
關(guān)注我們
歡迎您關(guān)注我們的微信公眾號了解更多信息:
歡迎您關(guān)注我們的微信公眾號
了解更多信息
国产精品久久久久日本影院| asian艳丽的少妇pics| 国产熟女一区二区三区五月婷| 三个男人的共妻每天被c| 88国产精品视频一区二区三区| 国产69精品久久久久久人妻精品| 亲子乱av视频一区二区| 国精产品一区一区三区有限公司| 人与禽120分钟性视频| 教官趁她睡着吸允她的花蜜| 奶头被民工吸的又大又黑 | 艳肉观音性三级DVD| 被全班玩弄的小柔h| 丰满白嫩少妇肉肉大hd| 回民丰满少妇xxx性| 又大又长粗又爽又黄少妇视频| 日本巜侵犯人妻人伦| 日本三级在线播放| 欧美av在线观看| 特大欧美黑人巨大XXOO| 成人三级做爰视频在线看| 人妻女教师耻辱の教室| 少妇aaa级久久久无码精品片 | 日本漫画工囗全彩内番漫绅士| 亚洲一区二区嗯好爽快点| 午夜精品一区二区三区在线观看| 黑人勃起太大进不去| 国产成人一区二区三区传媒| 狠狠躁夜夜躁人爽碰88a| 久艾草久久综合精品无码国产| 免费a级毛片无码免费视频120软件 | 他用嘴巴含着我奶头吸怎么办| 国产毛多女人视频| 国模无码视频一区二区三区| 欧美熟妇丰满肥白大屁股免费视频| 粗暴调教玩弄np高h小说| 精品无人乱码一区二区三区| GAY国产GV又粗又长又大| 奇米777四色777欧美在线| 亚洲中文无码| 337p粉嫩日本亚洲大胆艺术|